| Level | Preview | Description |
|---|
| Monkeying Round and Round | A forest level with many ropes and Ukikis. |
| Lakitu Peekaboo | An underground level with many Wall Lakitus. |
| Rollin' Down the River | A rapids level that features Lunge Fish and paddle wheels. |
| Knot-Wing the Koopa's Aqua Fort | A fortress level where Knot-Wing the Koopa is fought a second time. |
| Sunset at Curtain Falls | A level where Yoshi must ride curtains and avoid Fangs. |
| Spooky Scraps! Don't Get Spooked! | A level where certain shades reveal platforms and beads but make enemies invincible. |
| Yoshi Branches Out | A forest level where Yoshi must utilize certain moving arrow circles to advance. |
| Naval Piranha's Sewer | A sewer castle level where Naval Piranha is fought. |
| Naval Piranha 2: Now It's Personal! | A special level in which Naval Piranha chases the player. |